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lyso flipper tr  (Spirochrome)


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    Spirochrome lyso flipper tr
    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with <t>Lyso</t> Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
    Lyso Flipper Tr, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lyso flipper tr/product/Spirochrome
    Average 94 stars, based on 7 article reviews
    lyso flipper tr - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics"

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics

    Journal: Science Advances

    doi: 10.1126/sciadv.ads6132

    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
    Figure Legend Snippet: ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.

    Techniques Used: Fluorescence, Staining



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    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with <t>Lyso</t> Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
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    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with <t>Lyso</t> Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
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    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with <t>Lyso</t> Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.
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    Image Search Results


    ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.

    Journal: Science Advances

    Article Title: External strain on the plasma membrane is relayed to the endoplasmic reticulum by membrane contact sites and alters cellular energetics

    doi: 10.1126/sciadv.ads6132

    Figure Lengend Snippet: ( A ) Schematic diagram illustrating the experimental approach used to mechanically stimulate monolayers of mouse tendon cells. ( B ) Representative FLIM images of PM tension probed by Flipper-TR in tendon cells with or without cyclic mechanical stimulation. Each bottom panel is an enlargement of the dashed boxed area in the corresponding top panel. ( C ) Distribution of fluorescence lifetime of Flipper-TR after selecting the PM as the region of interest (ROI) between the static group and the stretched group ( n = 25 cells per group, each with at least two ROIs, from three independent experiments). ( D ) Representative FLIM images of overall ER (top) stained with ER Flipper-TR in tendon cells with or without cyclic mechanical stimulation and their enlargements showing ER tubules (middle), as indicated by the red arrows in the top images, and areas of ER sheets (bottom), as indicated by purple dashed rectangles in the top images. ( E ) Distribution of fluorescence lifetime of ER Flipper-TR selecting the overall ER, ER tubules, or ER sheets, as the ROI between the static group and the stretched group ( n = 24 cells per group, each with at least two ROIs, from three independent experiments). ( F ) Representative FLIM images of mitochondria stained with Mito Flipper-TR (top) and lysosome stained with Lyso Flipper-TR (bottom), in tendon cells with or without cyclic mechanical strain. Each top right panel is an enlargement of the red dashed boxed area in the yellow dashed boxed bottom left panel. ( G ) Distribution of fluorescence lifetime of Mito Flipper-TR and Lyso Flipper-TR between static group and stretched group ( n = 30 cells per group, each with at least three ROIs, from three independent experiments). Line marks the mean of the distribution. Scale bars, 1 μm. ** P < 0.01; *** P < 0.001; n.s., not significant by Student’s t test.

    Article Snippet: Samples were then incubated with 1 μM Flipper-TR (SC020, Spirochrome), 1 μM ER Flipper-TR (SC021, Spirochrome), 1 μM Mito Flipper-TR (SC023, Spirochrome), or 1 μM Lyso Flipper-TR (SC022, Spirochrome) for 15 min and washed with live-cell imaging solution (A14291DJ, Invitrogen) before imaging.

    Techniques: Fluorescence, Staining